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. 2005 Jul 18;170(2):225–235. doi: 10.1083/jcb.200504140

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — Low expression levels of MLP2 in conjunction with spc110-220 cause lethality and loss of nuclear integrity at 37°C. (a) Two individual clones of strains expressing either SPC110 or the spc110-220 allele alone (−) or in combination with either MLP1 or MLP2 under the control of the GAL1-10 promoter (Gal-MLP1 and Gal-MLP2, respectively) were spotted in 10-fold dilution steps on dextrose-containing plates and grown for 2 d at the indicated temperatures. (b) DAPI-stained cells of the indicated genotypes were scored for grossly aberrant nuclear morphology (n > 300). (Inset) Typical appearance of cell with altered nucleus. (c) Semiquantitative relative measure of dilution-adjusted colony density from strains with the indicated genotype and their counterparts carrying the wild-type SPB protein allele grown as described in panel a. This analysis was performed as previously described (Tackett et al., 2005). Data presented in panel a and in Fig. S4 was used for this analysis.