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. 2005 Jul 18;170(2):273–283. doi: 10.1083/jcb.200502055

Figure 7.

Figure 7.

Rescue of the growth and secretion defects of sec3 Δ by overexpression of SRO7 and SSO2. (A) Wild-type and sec3Δ haploid strains carrying SSO2 or SRO7 2μ plasmids, or the empty vector, were grown on SC plates at 25°C (left) and 34°C (right). SSO2 or SRO7 high-copy plasmids partially rescue the growth of sec3Δ cells. (B) Growth of indicated strains at 25°C was assayed by measuring the OD (OD600) after dilution from overnight culture in SC medium. Doubling times of cells at the exponential growth phase were measured (n = 3). (C) Invertase secretion defects were measured in wild-type, sec3Δ, and sec3Δ strains transformed with 2μ SRO7 or SSO2 in SC medium at 25 or 34°C. Accumulation of intracellular invertase was expressed as internal invertase/(internal invertase + external invertase). n = 3. Error bars represent SD.