Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Sep 12;170(6):983–992. doi: 10.1083/jcb.200503113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Dll3 does not activate N signaling in trans. (A) Cell surface expression of HA-tagged Dll1, Dll3, and Dll1 + Dll3 L cell lines was determined by biotinylation, streptavidin (SAV) pull-down, and blotting with anti-HA mAb (12CA5). (B) NIH 3T3 cells transfected with N and CSL reporter were cocultured with J1, Dll1, Dll3, mDll3, or parental (L) cell lines and were assayed for luciferase activity. Error bars reflect the SD of the mean from three experiments. RLU, relative luciferase units. (C) Parental C2C12 and cell lines expressing N1 were cocultured with L, J1, Dll1, or Dll3 cells, and myogenesis was monitored by myosin light chain 2 (MLC2) mRNA expression. Loading and transfer of RNA was monitored by methylene blue staining of 18S rRNA. (D) NIH 3T3 cells transfected with N1, CSL reporter, and control or LFng were cocultured with Dll1, J1, rDll3, mDll3, or L cells and were assayed for luciferase activity (n = 3). (E) Parental L, Dll1, or Dll1 + Dll3 cells were cocultured with NIH 3T3 cells transfected with CSL reporter and N or vector. There was no statistically significant difference between Dll1 and Dll1 + Dll3 (n = 3).