Table II.
t 1/2 values for FRAP experiments
| Strain | Growth conditionsa | GFP-tagged protein | Mean t 1/2 recoveryb (s) |
|---|---|---|---|
| YMK880 | Control | eIF2Bγ-GFP | RI |
| YMK883 | Control | eIF2α-GFP | 3.63 ± 0.60 |
| YMK883 | −AA (15 min) | eIF2α-GFP | 13.45 ± 1.87 |
| YMK883 | −AA (1 h) | eIF2α-GFP | RI |
| YMK1088 (gcn2Δ) | −AA (1 h) | eIF2α-GFP | 3.35 ± 0.29 |
| YMK883 + pRS316 | Control | eIF2α-GFP | 3.66 ± 0.36 |
| YMK883 + pAV1245, GCN2c | Control | eIF2α-GFP | RI |
| YMK883 + pAV1248, GCN2c | Control | eIF2α-GFP | 17.31 ± 2.30 |
| YMK1168 eIF2Bɛ (wt) | Control | eIF2α-GFP | 4.35 ± 0.63 |
| YMK1169 eIF2Bɛ (F250L) | Control | eIF2α-GFP | RI |
a
Control. Cells were grown to exponential phase in SCD or SCD minus LEU at 30°C except in –AA, where all amino acids were removed for the indicated time.
b
Mean value was calculated from at least 10 different FRAP experiments. The values are shown ± the standard error.
RI, recovery insufficient for t 1/2 measurement.