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. 2005 Aug 1;170(3):349–355. doi: 10.1083/jcb.200504028

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Reduced NAD levels in degenerating axons. (A) HPLC chromatograms of the axonal segments undergoing Wallerian degeneration. DRG explants cultured for 5 d were subjected to axonal transection, and the acidic extracts of axonal segments were prepared at the indicated time-points after transection and analyzed by HPLC. The chromatograms containing NAD and a few other pyridine compounds, including IMP, AMP, and nicotinic acid (Na) plus GDP from the same amounts of axonal extracts are shown. The absorbance (mAU) was monitored at 260 nm. (B) Quantification of NAD levels in control and transected axons. NAD levels were normalized against total axonal protein contents and expressed as nmol/mg. The results are presented from six independent experiments. Statistical analysis was done by t test. ***, P < 0.001. (C) Representative phase images of axons undergoing Wallerian degeneration taken at indicated time-points after transection. Bar, 50 μm. (D) Quantification of axon degeneration results. Statistical analysis was done by t test. ***, P < 0.001.