Figure 7.

Nuclear lysates of NGF-deprived sympathetic neurons show additional antiphospho-c-Jun Ser73–immunoreactive proteins. 5-DIV–sympathetic neurons isolated from P0 rats were deprived of NGF (−NGF) or left untreated (NGF) for 24 h. Nuclear and postnuclear extracts were subjected to SDS-PAGE, followed by Western analysis with phospho-c-Jun Ser73 antiserum. Arrow indicates the c-Jun protein. Stars show the antiphospho-c-Jun–immunoreactive proteins detected in whole cell extracts (see Fig.8). The immunoreactive bands seen only in the nuclear fraction are indicated by arrowheads. Panel on the left is the longer exposure of the nuclear fraction part. Phospho-c-Jun signal (arrow), present mainly in the nuclear fraction, shows that this was a crude subcellular fractionation of the sympathetic neuron nuclei. Equal amounts of protein were loaded in each lane (unpublished data).