Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Aug 15;170(4):537–549. doi: 10.1083/jcb.200502154

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 10. — Inhibition of histone deacetylase activity increases the dynamics of both SUV39H1 and HP1β. NIH3T3 cells were transfected with EYFP-SUV39H1 (A) or EYFP-HP1β (B) and treated with 50 ng/ml TSA. After 18–22 h of TSA treatment, a heterochromatic area was selected and photobleached. Images were recorded just before and at different time intervals after photobleaching. Arrows indicate the photobleached areas. (C) The corresponding FRAP curves are plotted together with the FRAP curves for EYFP-SUV39H1 and EYFP-HP1β obtained from nontreated cells. Curves represent means from 22 and 8 cells, respectively. (D) Western blots showing the levels of acetylation and trimethylation in nontreated (lane 1), TSA-treated (lane 2), and 5-aza-C–treated (lane 3) NIH3T3 cells. Ponceau staining shows equal protein loading.