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. 2005 Aug 15;170(4):537–549. doi: 10.1083/jcb.200502154

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — EYFP-SUV39H1 expression rescues the phenotype of Suv39h dn PMEFs. Suv39h dn and wild-type PMEFs transfected with EYFP-SUV39H1 were labeled with antibodies against trimethylated H3K9 (A) or HP1β (B). In nontransfected Suv39h dn PMEFs, a little trimethylated H3K9 is present throughout the nucleus (A, arrows). Transfected cells show an increase in trimethylated H3K9 that is localized to heterochromatic areas (A, middle and bottom). This localization is comparable to wild-type cells (A, top). Bars, 5 μm.