Skip to main content
. 2005 Aug 15;170(4):607–618. doi: 10.1083/jcb.200505128

Figure 1.

Figure 1.

PtdIns(3)P is enriched in the Rab5 domain. (A) NIH3T3 cells expressing Rab5(Q79L) and GFP-tagged 2XFYVE, Akt PH domain, FAPP1 PH domain, or PLCδ PH domain were stained with antibodies against Rab5 (4F11) followed by rhodamine-conjugated secondary antibodies. Bar, 10 μm. A431 cells were microinjected with myc-2XFYVE, CFP–Rab5, and YFP–Rab4 (B) or myc–2XFYVE, YFP–Rab4, and CFP–Rab11 (D) and stained with antibodies against myc (9E10) and Texas red–conjugated secondary antibodies. The extent of colocalization between 2XFYVE, Rab5, and Rab4 (C) or 2XFYVE, Rab4, and Rab11 (E) was quantified. Colors in the histogram correspond to those used in the merge of double or triple fluorescent signals in the panels. White arrows indicate positive for 2XFYVE, Rab5, and Rab4. Green arrows indicate positive for Rab4 but negative for 2XFYVE and Rab5. Bar, 2 μm.