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. 2005 Aug 15;170(4):607–618. doi: 10.1083/jcb.200505128

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — Knockdown of 4-Pase decrease transferrin uptake. (A) Reduced levels of 4-Pase 72 h after transfection of HeLa cells with siRNA, as detected by Western blot. (B) Cells transfected with unspecific (diamonds) or 4-Pase–specific (triangles) siRNA were allowed to internalize biotinylated transferrin for the indicated times. The internalized transferrin was quantified as described in Materials and methods, and standardized with respect to total protein concentration of the lysate and expressed as percent of the amount of internalized transferrin in control cells at t = 40 min (n = 4; mean ± SD of two independent experiments).