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. 2005 Aug 15;170(4):527–535. doi: 10.1083/jcb.200505075

Figure 3.

Figure 3.

orc3 is required for dendritic growth and branching. (A) Northern analysis of mouse orc3 expression in COS cells transfected with control (lane C) or orc3 siRNA constructs (lanes 1–8). (B) Western analysis of mouse Orc3 protein expression in COS cells transfected with control (C) or orc3 siRNA constructs (1, 1m, and 4). (C–C′) Confocal micrographs of hippocampal neurons stained for Orc3 (B′, red). A siRNA1-transfected neuron was labeled by EGFP (B, green). Notice the loss of Orc3 staining from the transfected cell (arrowhead). (D–F) Confocal micrographs of 10 DIV neurons transfected on 7 DIV with control (C), siRNA1 (D), or siRNA4 (E) constructs and EGFP (green). (G–G″ and H–H″) Confocal micrographs of neurons transfected with control (G–G″) or siRNA1 constructs (H–H″) and stained for EGFP (green) and MAP2 (red). (I–L) Quantitative analysis of dendritic morphology. Total dendritic length of neurons transfected with orc3 (siRNA1, n = 26, and siRNA4, n = 24) or orc5 (siRNA11, n = 24) siRNA or mutated siRNA1 (siRNA1m, n = 17) is compared with control (n = 27, all ≥3 experiments) neurons (I). Total branchpoint numbers (J), branchpoint distribution (K), and density (L) are also compared. Error bars reflect SEM. *, P < 1.0 × 10−6 (all compared with control). #, P > 0.28 (compared with control); P < 1.0 × 10−9 (compared with siRNA1). Bars, 50 μm.