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. 2005 Aug 15;170(4):527–535. doi: 10.1083/jcb.200505075

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — orc4 ATPase motif mutants promote dendritic branching. (A–C and A′–C′) Confocal micrographs of 7 DIV neurons transfected on 0 DIV with wild-type (O4WT, A and A′) or mutant (O4EQ, B and B′; O4KA, C and C′) orc4 constructs and EGFP (green). Neurons were also stained for MAP2 (red). (D–F) Quantitative analysis of dendritic morphology. Differences are not observed between O4WT (n = 49) and O4EQ (n = 43) or O4KA (n = 27) in total dendritic length (D), but found in total branchpoint numbers (E) and branchpoint density (F). Error bars reflect SEM. *, P < 0.008; **, P < 0.002 (all compared with O4WT). Bar, 50 μm.