Table I. Equilibrium dissociation constants (Kd) for interactions among Myo1p tail, Wsp1p VCA, verprolin, Arp2/3 complex, and actin.
| Receptor | Ligand | Binding | Kd (μM) |
|---|---|---|---|
| GST-Myo1p-23A | Arp2/3 complex | + | 1.2/1.8 |
| GST-Myo1p-3A | Arp2/3 complex | + | 2.0/2.1 |
| GST-Myo1p-23 | Arp2/3 complex | − | |
| GST-Wsp1p-VCA | Arp2/3 complex | + | 0.05 |
| GST-Myo1p-23A | Verprolin | + | 4.6/5.6a (3–6 μM) |
| GST-Myo1p-3A | Verprolin | + | N.D. |
| GST-Myo1p-23 | Verprolin | + | N.D. |
| Vrp1p-His | G-actin | + | 1.0/0.9/0.8 |
Equilibrium dissociation constants were measured using quantitative pull-down assays. Slashes separate values from independent experiments. N.D., not determined.
a
Values represent the best fits, the range of values from all fit attempts is in parentheses.