Figure 1.

GDAP1 protein is expressed in Schwann cells and neurons. (A) 15 μg of protein lysate from the indicated neuronal tissues was analyzed by Western blot. In all tissues tested, GDAP1 was detected at the predicted size of 41.5 kD (arrowhead). The expression was highest in central nervous system tissues. The additional weak bands at ∼48 kD and ∼75 kD were not detected with another GDAP1 antiserum, and thus, were considered to be unspecific. (B) GDAP1 expression on cryosections of rat sciatic nerve. The signal colocalizes on single-plane confocal pictures (0.5-μm sections) with the Schwann cell marker S100 (1B, a–c) and the neuronal marker neurofilament (1B, d–f). Bars, 10 μm.