Figure 3.

Maskin depletion impairs centrosome aster formation in egg extract. (A) Representative images of asters by purified centrosomes in mock-depleted extract (Mock), Maskin-depleted extract (ΔMaskin), and Maskin-depleted extract containing purified GST-Maskin protein (AdB). The extract was sent to interphase and cycled back into mitosis by addition of cyclin B Δ90. Bar, 10 μM. (B) Graphs showing the average microtubule fluorescence intensity (left) and microtubule length (right) in asters assembled under the three conditions as in A: mock (white bars), Δ Maskin (dark gray bars), Add-Back (light gray bars). (C) Western blot of the egg extracts used in A and B probed with anti-Maskin and anti-tubulin antibodies.