Figure 6.

Synapse size, but not number, is altered in neurons lacking MALS. Autaptic cultures (14 DIV) from control and TKO mice were stained with antibodies to PSD-95 and synaptophysin. (A) Representative autaptic neurons from WT and TKO mice stained with synaptophysin. Bars: (top) 20 μm; (bottom) 5 μm. (B) PSD-95/synaptophysin staining revealed that the number of synapses was unaltered in TKO cultures (0.65 ± 0.15 and 0.67 ± 0.18 synapses/μm for WT and TKO, respectively). (C) As determined by synaptophysin staining, the distribution of presynaptic terminal size was shifted to the right in the TKO (P < 0.01). All error bars represent SEM.