Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Sep 26;170(7):1091–1099. doi: 10.1083/jcb.200507048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — An intact DHHC-CRD is required for PFA3 to function in vacuole fusion. (A) pfa3Δ cells transformed with vector (pRS313), PFA3-2xFlag (pML780), or C134S PFA3-2xFlag (pML782), were grown in selective media, treated with 2 mM DTT, and stained with FM 4–64 before microscopy. Pictures are representative of two independent transformants. Bars, 2 μm. (B) Whole cell lysates (50 μg) from pfa3Δ transformants used in A were probed with α-Flag antibody to detect expression of Pfa3p-2xFlag.