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. 2005 Sep 26;170(7):1091–1099. doi: 10.1083/jcb.200507048

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Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — Pfa3p promotes Vac8p palmitoylation in membranes. Pfa3p-Flag (WT [pML395] or C134S[pML724]) and Vac8p-myc (WT [pML734] or Cys⁻ [pML775]) constructs were coexpressed in yeast and membranes incubated with [³H]palm-CoA for 10 min and analyzed by fluorography or subjected to Western blot to evaluate protein expression. The result shown is representative of four experiments. The radiolabeled samples were exposed to film for 10 d.