Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Sep 26;170(7):1091–1099. doi: 10.1083/jcb.200507048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Partially purified Pfa3p-Flag autoacylates and palmitoylates Vac8p. Detergent extracts from Pfa3p-Flag–expressing cells or vector-transformed cells (pESC) were purified by Flag-immunoaffinity chromatography. Equal volumes of Pfa3p-Flag and pESC elutions were analyzed by (A) silver stain, (B) α-Flag Western blot, or (C) fluorography after incubation with [³H]palm-CoA. (D) Pfa3p-Flag (0.5 pmol) and myr-Vac8p-myc-6xHis (0.4 μg total protein/3 pmol Vac8p) were incubated with [³H]palm-CoA for the indicated time and analyzed by fluorography (inset; 4 d exposure) and scintillation counting (curve). Data are representative of two independent experiments.