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Purification of Flag-Pfa3p
| Protein | Total Activity | Specific Activity | Purification | |
|---|---|---|---|---|
| mg | U | U/mg | fold | |
| Pfa3p | ||||
| Membranes | 10.0 | 88.0 | 8.8a | 1.0 |
| Extract | 6.0 | 64.0 | 10.7 | 1.2 |
| Elution | 0.005 | 1.6 | 320.0 | 36.0 |
| pESC | ||||
| Membranes | 10.0 | 1.2 | 0.12 | |
| Extract | 8.3 | 1.0 | 0.12 | |
| Elution | 0.001 | 0 | 0 |
One unit of activity is pmol of [3H]palm-Vac8p produced per minute.
