Figure 1.

ERG phenotype and expression of TRP in trp¹⁴ flies. (A–F) ERG recordings. Flies <1 d after eclosion were dark-adapted for 2 min before exposure to a 10-s pulse of orange light (indicated by the event marker below the ERGs). (A) Wild type (wt); (B) trp ^P343; (C) trp ¹⁴; (D) trpl ³⁰²; (E) trpl ³⁰²;trp ^P343; (F) trpl ³⁰²;trp ¹⁴. (G) Time required for 60% return to the dark state. Shown are the mean times in seconds (s) based on ERGs obtained from ≥20 flies of the indicated genotypes. SDs are indicated. (H–J) Photoreceptor cell responses assayed by intracellular recordings. 1-d-old flies were dark adapted for 2 min before being exposed to a 10-s pulse of orange light. Scale bars are provided indicating the amplitude (mV) and timescale (s). (H) wt; (I) trp ^P343; (J) trp ¹⁴. (K) Expression of TRP in mutant alleles assayed on Western blots. Head extracts were prepared from flies <1 d after eclosion and a Western blot was first probed with anti-TRP antibodies and subsequently with anti-NORPA antibodies to provide a loading control. The positions of protein size markers (kD) are indicated. (L) Expression levels of core members of the signalplex in trp ¹⁴. The Western blot was first probed with anti-TRP antibodies and then reprobed with anti-INAD, anti-NORPA, anti-INAC, and anti-tubulin antibodies. (M) Relative TRP protein levels in trp ¹⁴ flies. The TRP protein level in trp ¹⁴ heads was compared with wt, trp ^P343, and trp ¹⁴ heterozygotes. The intensities of the bands were determined using a phosphoimager. SDs are indicated.