Figure 3.

Expression of low levels of TRP rescues the trp ERG phenotype. (A) Expression of hs-trp results in expression primarily in the retina. Newly eclosed hs-trp,trp ^P343 flies were heat shocked for 2 h at 37°C and frozen sections, which were prepared 24 h later, were stained with anti-TRP antibodies. cb, central brain; la, lamina; me, medulla; re, retina. (B) Western blot showing TRP protein levels in hs-trp,trp ^P343 flies exposed to heat shock (hs) treatment of the indicated durations. The heat shocks were performed on newly eclosed flies and the head extracts were prepared 24 h later. The second lane from the left contains 10% the quantity of extracts (from wild-type fly heads) loaded in the other lanes. The same blot was reprobed with anti-tubulin antibodies. (C) Relative levels of TRP expressed in trpl;hs-trp,trp ^P343 flies exposed to different heat shock durations. The TRP protein levels after different heat shock times were compared with the band intensity obtained using 10% as much wild-type extracts. The quantification was performed using a phosphoimager. SDs are indicated. (D–G) ERG recordings obtained in trpl;hs-trp,trp ^P343 flies exposed to heat shock treatments of different lengths. Flies were heat shocked for either 0.5, 1, or 2 h at 37°C immediately after eclosion and the ERGs were performed 1 d later by dark adapting the flies for 2 min and exposing them to a 10-s pulse of orange light.