Figure 1.
HC11 cells form polarized, growth-arrested acini when cultured on a basement membrane matrix. Representative confocal microscopic images are shown. Day 6 HC11 acini were immunostained with antiserum to p-histone H3 (A, green) and α6 integrin (A, red) or p-ERM (B, green). Day 8 HC11 acini were immunostained with antiserum to p-histone H3 (C, green) and α6 integrin (C, red), p-ERM (D, green), cleaved caspase 3 (E, green) and α6 integrin (E, red), and laminin V (F, green) and cleaved caspase 3 (F, red). Day 10 HC11 acini were immunostained with antiserum to α6 integrin (G, red) and p-ERM (G, green) and to laminin V (H, green). Bars, 10 μm.