Table II. wun-2 expression in the CNS can rescue lateral migration of PGCs in wun/wun-2 mutants.
| Stage 11
|
Stage 13
|
|||||
|---|---|---|---|---|---|---|
| Genotype |
PGCs in the middle of the embryoa (%) | Total number of PGCs |
n
|
PGCs in the gonad (%) | Total number of PGCs |
n
|
| wunGL, UAS-wun-2/wunGL | 20.5 ± 0.6 (52.2) | 43.1 ± 0.9 | 26 | 6.4 ± 0.8 (17.0) | 37.6 ± 1.6 | 21 |
| wunGL, UAS-wun-2/wunGL, 9-201av-Gal4 | 17.5 ± 0.6 (39.0) | 44.9 ± 1.3 | 27 | 19.1 ± 1.1 (48.1) | 39.7 ± 1.1 | 23 |
| wunGL, UAS-wun-2/CyO or wunGL/CyO | 3.5 ± 0.5 (9.3) | 37.6 ± 0.7 | 33 | 26.4 ± 0.8 (89.8) | 29.4 ± 0.8 | 30 |
Embryos were stained with anti-Vas antibody.
a
PGCs in the central one-third diameter of the embryo were counted.