Figure 6.

EphA4 interacted with ARMS, phosphorylated ARMS, and α-syntrophin. (A) Cell lysates were prepared from COS7 cells overexpressing ARMS and EphA4, were immunoprecipitated with ARMS 892 antiserum, and were immunoblotted with EphA4 antibody. (B) Reciprocal coimmunoprecipitation using EphA4 antibody. (C and D) In vivo coimmunoprecipitation was performed using 14 DIV cultured cortical neurons and membrane proteins from rat muscle, respectively. (E) ARMS and α-syntrophin were overexpressed in COS7 cells together with wild-type EphA4 or its KD mutant. Cell lysates were immunoprecipitated using ARMS antibody and were immunoblotted with ARMS, HA, or EphA4 antibody. (F and G) EphA4 tyrosine-phosphorylated ARMS and α-syntrophin in COS7 cells.