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. 2005 Jun 6;169(5):813–824. doi: 10.1083/jcb.200412008

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — ARMS and α-syntrophin enhanced the EphA4-induced Jak/Stat activation. (A) COS7 cells were transfected with EphA4, EphA4 KD mutant, or ARMS, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr^1007/1008 of mouse Jak2, Tyr^1054/1055 of human Tyk2, Tyr⁷⁰¹ of Stat1, and Thr²⁰²/Tyr²⁰⁴ of ERK1/2. p-Tyr; phosphorylated Tyr. (B) Quantification of the Western blots. y axis represents the fold change of tyrosine phosphorylation. n = 3; *, P < 0.05; **, P < 0.005. (C) COS7 cells were transfected with EphA4, ARMS, α-syntrophin, or α-syntrophin mPDZ, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr^1007/1008 of mouse Jak2, Tyr^1054/1055 of human Tyk2, Tyr⁷⁰¹ of Stat1, and Thr²⁰²/Tyr²⁰⁴ of ERK1/2. (D) Quantification of the Western blots. n = 3; *, P < 0.05; **, P < 0.005. Error bars represent the SEM.