Figure 5.

Hyperphosphorylated γ-tubulin in GPM40 complexes. (A) GPM40 complexes were prepared as described in Materials and methods. After 2D-gel electrophoresis, Western blots were performed with anti–γ-tubulin Ab. The background spots that were detected with the second Ab alone were used as internal markers to align γ-tubulin spots. Control: GPM40 complexes without phosphatase treatment. PPase: GPM40 complexes treated with phosphatase. (B) GPM complexes were prepared at 20, 40, 60, and 80 min after initiation of the differentiation. After 2D-gel electrophoresis and Western blotting with anti–γ-tubulin Ab, γ-tubulin spots were aligned as described above.