Table I.

Phosphatase inhibitors induce the production of multiple flagella ^{a b c d}

	Phosphatase inhibitor				Control
Time (min)	a	b	c	d	a	b	c	d
0	0	0	0	0	0	0	0	0
40	0	0	0	0	0	0	0	0
60	17 ± 0.7	2.3 ± 0.2	0	15 ± 2.7	13 ± 0.7	0	0	0
80	52.3 ± 1.6	10 ± 0.7	0.7 ± 0.2	18.2 ± 0.9	76.7 ± 0.2	1.3 ± 0.1	0	1.7 ± 0.3
100	59.3 ± 2.9	13.3 ± 0.2	1.6 ± 0.2	20.8 ± 0.3	88.3 ± 0.9	2.3 ± 0.2	0	2.6 ± 0.2
120	64 ± 0.7	16.3 ± 0.7	2.3 ± 0.2	22.5 ± 0.5	95.3 ± 0.9	3.3 ± 0.2	0	3.4 ± 0.3

N. gruberi amoebae were divided into two flasks and differentiated at the same time. At 50 min after initiation, PI were added into one of the flasks. At 20-min intervals, 200 μl of differentiating cells were taken, fixed with Lugol's iodine, and observed under phase contrast optics. The percentage of flagellated cells and the number of flagella per flagellated cell were determined by randomly counting 100 cells at each time point. The numbers in Table I are the average of three independent experiments.

^aThe percentage of cells with one pair of flagella.

^bThe percentage of cells with two pairs of flagella.

^cThe percentage of cells with three (or more) pairs of flagella

^dThe percentage of cells with excess flagella (b + c)/(a + b + c).