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Overexpression of human and zebrafish Gα12/13 promoted stress fiber formation. Stress fiber formation was determined in HEK 293 cells transiently transfected with cDNAs encoding GFP (A and B), WT human (C) or zebrafish (E and F), constitutively active human (D) or zebrafish (G and H) G-proteins. Cells were stimulated with (B) or without (A, and C–H) 10 nM thrombin for 10 min, and then stained with 0.17 μM Rhodamine-phalloidin. 10 μM Y-27632 was used to block Rho kinase activity (H).
