Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jun 6;169(5):725–731. doi: 10.1083/jcb.200411001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Uncaging cGMP increases Ca² ⁺ in swimming sperm. (A and B) Fluorescence images of caged cGMP- and fluo-4–loaded sperm before and after UV flash in (A) ASW and (B) ASW containing 30 μM nimodipine. Interval between images = 23 ms. Bars, 20 μm. Color bar shows fluorescence intensity after background subtraction. (C–H) Normalized increases in fluo-4 fluorescence (F/F₀) in flagellum (black) and head (gray) of individual swimming sperm after uncaging of cGMP. Arrow with line denotes UV flash. (C and D) ASW, (E and F) ASW with 30 μM nimodipine, (G) ASW with 50 mM K⁺, (H) zero Ca²⁺ ASW. Video 1 shows animated sequence of images in A and B (Video 1 available at http://www.jcb.org/cgi/content/full/jcb.200411001/DC1).