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. 2005 Jun 6;169(5):725–731. doi: 10.1083/jcb.200411001

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Uncaging cGMP induces characteristic motility changes in single sperm. (a) Trajectory of an individual caged cGMP-loaded sperm in ASW before (red) and after (black) a UV flash. Points along trajectory trace are 23 ms apart. Position of the sperm at the moment of UV flash is marked by a white circle. Bar, 60 μm. Curved arrow shows sperm swimming direction. Graph below shows curvature of sperm trajectory over time. Arrow and solid line indicate UV flash. Dashed line = 0.05 radians/μm. (b–e) Sperm trajectories during a series of UV flashes (b) ASW, (c) ASW containing 30 μM nimodipine, (d) high K⁺ ASW, (e) zero Ca²⁺ ASW.