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. 2005 Jun 20;169(6):921–928. doi: 10.1083/jcb.200412167

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — FACS and microarray analysis of kidney SP cells. (A) Representative FACS profile of SP cells isolated from the kidney of normal (ddY and ICR) and renal failure (HIGA and ICGN) mice (total events:1.0 × 10⁵ to 3.0 × 10⁵). (B) Kidney SP cells isolated from ddY, ICR, HIGA, and ICGN mice stained with Hoechst dye (white bars). Total event of all mice were set 1.0 × 10⁵, and uptake of Hoechst dye was significantly blocked in the presence of reserpine (res) in all samples (black bars). Results are presented as the mean ± SEM, n = 15. *, P < 0.05 versus control (without reserpine). (C) Scatter-plot analyses of gene expression (3,800 genes) in SP cells versus kidney tissue prepared from normal (ddY and ICR) and renal failure (HIGA and ICGN) model mice. Average expression levels of each gene were calculated from three independent hybridizations.