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. 2005 Jun 20;169(6):921–928. doi: 10.1083/jcb.200412167

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — LIF-induced gene expression is regulated by musculin/MyoR. (A and B) Kidney SP cells (A) or non-SP cells (B) just after FACS sorting were incubated with vehicle (white bars), LIF (10 ng/ml; black bars), LIF with reserpine (10 ng/ml and 50 μM, respectively; dotted bars), and LIF with RA (10 ng/ml and 5 × 10⁻⁷ mol/l, respectively; hatched bars) for 12 h, and then gene expression of HGF, VEGF, and BMP7 was examined by TaqMan real-time PCR. (C and D) Gene expression of musculin/MyoR in kidney SP cells. An aliquot of RNA in panel A was examined by RT-PCR (C) and TaqMan real-time PCR (D). (E) Gene expression of musculin/MyoR in non-SP cells. Basal expression of musculin/MyoR was almost absent. Values represent means ± SEM (n = 6). *, P < 0.05 versus control; **, P < 0.05 versus LIF.