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. 2005 Jun 20;169(6):871–884. doi: 10.1083/jcb.200502088

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Role of eIF2α phosphorylation and Lsm4 expression in SG and PB formation. (A) Arsenite-treated wild-type (SS) and eIF2α S51A mutant (AA) MEFs stained for SG markers eIF3b, G3BP, and TIAR. (B) Arsenite-treated SS and AA MEFs stained for PB markers GW182 and DCP1a and the SG marker protein TIA-1. Yellow arrows indicate representative PBs; white arrowheads indicate SGs in the merged views. (C–E) DU145 or HT1080 cells were transfected with control siRNA or siRNA targeting Lsm4, processed for immunofluorescence, and examined for PBs and SGs. (C) Semiquantitative RT-PCR showing reduced expression of Lsm4 mRNA in Lsm4-siRNA–transfected HT1080 cells. (D) Percentage of cells containing visible PBs before (dark gray bars) or after (light gray bars) arsenite treatment. (E) Confocal micrographs of HT1080 cells stained for PB markers GW182 and DCP1a and SG marker TIA-1.