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. 2005 Jun 20;169(6):871–884. doi: 10.1083/jcb.200502088

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — SG and PB assembly induced by different stresses. HeLa cells were subjected to different stresses and were stained for eIF4E, FAST, and eIF3. (A) Unstressed cells, some of which contain PBs (yellow arrow) but no SGs. (B) Arsenite (500 μM for 30 min) induces both SGs (white arrowhead) and PBs (yellow arrow). (C) Cells were treated with arsenite for 60 min, and 20 μg/ml emetine was added during the last 30 min. (D–F) Cells were subjected to heat shock (44°C) for 15 min (C), 30 min (D), or 60 min (E). Yellow arrows indicate representative PBs; white arrowheads indicate SGs in the merged views. In each panel, the indicated inset is reproduced at the bottom as replicate views of the same field showing eIF4E, FAST, and eIF3.