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. 2005 Jun 20;169(6):885–896. doi: 10.1083/jcb.200409150

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Copyright © 2005, Government

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Figure 1. — Gβ1, Gβ2 and Rack1 interact with GR(263-419) in yeast two-hybrid assays. (A) Full-length Gβ1, Gβ2, and Rack1, as well as Rack1(139-317) interact with GR(263-420) in a yeast two-hybrid assay. EGY48 yeast cells were transformed with p8OP-LacZ, pLexA-GRα(263-419) and the indicated full-length Gβ1-, Gβ2-, Rack1-, or Rack1(139-317)-expressing pB42AD-derived plasmids. Bars represent mean ± SEM values of fold activation compared with the baseline. (B) Gβ2(143-270) (blades 3–5) interacts with GR(263-420) in a yeast two-hybrid assay. EGY48 yeast cells were transformed with p8OP-LacZ, pLexA-GRα(263-419) and the indicated Gβ2 fragment-expressing pB42AD plasmids. Bars represent mean ± SEM values of fold activation compared with the baseline. (C) Summary of yeast two-hybrid assays, which demonstrates domains of Gβ2 and Rack1 that are necessary for the interaction with GR(263-419).