Table I. FRAP of EGFP-CLASP2 on lamella MT lattices.
| k | Recovery | t 1/2 | n | |
|---|---|---|---|---|
| % | s | |||
| EGFP-CLASP2 | 1.21 ± 0.37 | 92.7 ± 5.1 | 0.63 ± 0.21 | 20 |
| towards −end of bleach zone | 1.25 ± 0.47 | 93.9 ± 4.0 | 0.62 ± 0.21 | 10 |
| center of bleach zone | 1.42 ± 0.45 | 91.5 ± 4.0 | 0.55 ± 0.23 | 10 |
| towards +end of bleach zone | 1.31 ± 0.38 | 92.7 ± 5.6 | 0.57 ± 0.17 | 10 |
| 3xEGFP-CLASP2 | 0.82 ± 0.30a | 86.5 ± 5.6 | 0.94 ± 0.29 | 20 |
| EGFP-CLASP2(340–1084) | 1.14 ± 0.31 | 95.7 ± 3.3 | 0.65 ± 0.18 | 20 |
| lithium chloride–treated | 0.74 ± 0.11a | 90.3 ± 2.5 | 0.96 ± 0.15 | 20 |
Values are mean ± SD. A subset of the EGFP-CLASP2 data was also analyzed at different positions along the MT in the bleached region to test for lateral transport. k, recovery rate constant; n, number of bleach events analyzed.
a
The recovery rate constants of 3xEGFP-CLASP2 compared to EGFP-CLASP2 and control compared to lithium chloride–treated EGFP-CLASP2(340–1084) were significantly different (P < 0.0001; t test).