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. 2005 Jan 3;168(1):141–153. doi: 10.1083/jcb.200405094

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Characterization of CLASP-specific antibodies and RNAi tools. (A) Lysates of HeLa cells, transfected with GFP-CLASP1α (lane 1), GFP-CLASP2α (lane 2), or mock transfected (lane 3), were analyzed by Western blotting with the indicated antibodies. (B) Lysates of HeLa cells, transfected with the indicated siRNAs, were prepared 24, 48, or 72 h after transfection and analyzed by Western blotting with the indicated antibodies. (C and D) Lysates of HeLa cells, transfected with the indicated siRNAs, were prepared 72 h after transfection and analyzed by Western blotting with the indicated antibodies. (E) HeLa cells, transfected either with the control siRNA, CLASP1+2#A or #B siRNAs, were stained with the mixture of antibodies #402 or #2358 72 h after transfection and analyzed by FACS. (F) HeLa cells were stained with a mixture of antibodies #402 (CLASP1) and #2358 (CLASP2) and either α-tubulin or the Golgi marker GM130. Bars, 10 μm. (G) HeLa cells, transfected either with the control siRNA or the CLASP1+2#B siRNAs, were stained with antibodies #402 or #2358 72 h after transfection. Bars, 10 μm.