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. 2005 Jan 3;168(1):89–101. doi: 10.1083/jcb.200408155

Figure 4.

Figure 4.

Inhibition of MLV release by WWP1 fragments. (A) Increasing amounts of the indicated YFP-WWP1-derived expression plasmids were transfected in 293T cells and fusion protein expression was monitored by Western blotting with an αGFP antibody. (B) Effect of YFP-WWP1 fragments on infectious wild-type MLV or MLV/HIVp6 release, measured by cotransfecting MLV proviral plasmids with increasing amounts of the indicated YFP-WWP1-derived expression plasmids. Infectious MLV virion production was measured as in Fig. 1. The assay background is indicated as a dotted line. Error bars indicate the SD from the mean of triplicate measurements. (C) Western blot analysis, using an αMLV Gag antibody, of cell lysates and extracellular MLV virions, after transfection of 293T cells with wild-type MLV or MLV/HIVp6 proviral plasmids and 200 ng of the indicated YFP-WWP1 expression plasmid derivatives. (D) Galleries of electron microscopic images of arrested and aberrant budding structures observed in 293T cells transfected with the MLV/dPY proviral plasmid or a wild-type MLV proviral plasmid and plasmids expressing the indicated YFP-WWP1 fragments.