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. 2005 May 23;169(4):591–601. doi: 10.1083/jcb.200412139

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — FIAT protein. (A) Amino acid sequence of the human FIAT protein. The putative leucine zipper is shaded in gray. (B) Nuclear extracts from MC3T3-E1 osteoblastic cells at confluence (lane 1) or after 6 d of growth in the presence of ascorbic acid and β-glycerophosphate (lane 2) were probed with the anti-FIAT peptide antibody. The arrow identifies the 66-kD FIAT protein. (C) Calvarial osteoblasts from primary cultures were stained with the anti-FIAT antibody. The endogenous FIAT protein is detected in the nucleus of the cells.