Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 May 23;169(4):591–601. doi: 10.1083/jcb.200412139

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — FIAT transgenic mice. (A) Schematic representation of the Col 1-FIAT transgene. Poly A, SV40 polyadenylation signal. (B) RT-PCR assay of transgene expression. Top panel shows specific FIAT transgene expression using primers from the FIAT sequence and the SV40 polyadenylation sequence. Bottom panel shows GAPDH expression. He, heart; Ki, kidney; Te, tendons; Br, brain; Mu, skeletal muscle; Sk, skin, Li, liver; Bo, bone. (C) Endogenous FIAT and transgene expression in osteoblasts and bone. RT-PCR with primers for endogenous FIAT (lanes 1 and 2) or primers specific for the transgene (lane 3). RNA was from osteoblast primary cultures (lane 1) or calvaria. Wt, wild type; Tg, transgenic. (D) Reduced bone mass in FIAT transgenic mice. Goldner (left) and von Kossa (right) stains of tibial sections from FIAT transgenic mice (bottom) and wild-type littermates (top) at 3 mo old. Note the smaller trabeculae and reduced secondary center of ossification in transgenic animals. Bar, 500 μM.