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. 2005 May 23;169(4):591–601. doi: 10.1083/jcb.200412139

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — Reduced activity of FIAT transgenic osteoblasts. Primary cultures of osteoblasts were obtained from 6–8-d-old calvaria (A and B) or 3-mo-old bone marrow stromal cells (C and D). Cells were assayed for AP level after 7 d or stained with alizarin red after 14 d. Number of samples assayed is indicated within each bar. AP concentration was normalized by protein concentration per well (A and C). Dark red mineralized nodules were expressed per squared millimeter (B and D) and cells were photographed with bright-field microscopy (E, wild type; F, transgenic). *, P < 0.05; **, P < 0.01.