Figure 3.

ATF6 is important in caspase-12 activation during myotube formation. (A) Activation states of ER stress sensors. Western blot analysis of myoblast cells cultured in DM for 24 h. L, live cells; D, dying cells. As positive controls, cells treated with either 2 μg/ml tunicamycin or 1 μM thapsigargin for 24 h were included in the analysis. (B) AEBSF inhibits apoptosis in C2C12 cells in DM. Proliferating cells were pretreated with 400 μM AEBSF or 100 μM p-APMSF for 1 h, and then transferred to DM in the presence of inhibitor for 24 or 48 h. Bars, 200 μm. (C) AEBSF treatment prevents induction of CHOP and myosin. Cells were immunostained by anti-CHOP antibody and antimyosin antibody. Bars: (CHOP) 50 μm; (Myosin) 100 μm.