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. 2005 Jan 31;168(3):389–399. doi: 10.1083/jcb.200409174

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — NBD-labeled translocation intermediates engaged with KRMs, XRMs, or RRMs. (a) Short nascent chains (e.g., pPL₆₄) are sealed off from the lumen by the BiP-mediated gate (hatched oval), whereas longer nascent chains (e.g., pPL-sK₇₈) are exposed to the lumen. Probe positions are indicated by open circles and the nascent chain within the ribosome tunnel is depicted as a dashed line. (b–d) Mean K_SV values (n = 3–12 ± SD) are shown for NBD-pPL₆₄ (b) or NBD-pPL-sK₇₈ (c and d) translocation intermediates functionally engaged with KRMs, XRMs, or RRMs as indicated. For RRMs, reconstitutions were performed either in the presence of ATP (b and c) or ADP (d). Measurements were made in the absence (•, I⁻ on cytosolic side of sealed membranes only) or presence (○, I⁻ both on cytosolic side and in lumen) of melittin.