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. 2005 Jan 31;168(3):389–399. doi: 10.1083/jcb.200409174

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Dependence of translocon gating on the SBD of BiP. (a) Mean fluorescence anisotropy values of NBD-pepCL9C (n = 3–5 ± SD) preincubated with increasing concentrations of rBiP (WT or 44K) with ATP, ADP, and unlabeled pepCL9C (fourfold molar excess) as indicated. (b) Mean K_SV values (n = 3 ± SD) for NBD-pPL-sK₇₈ associated with RRMs reconstituted with rBiP 44K or full-length rBiP (WT or T37G) measured in the absence (•) or presence (○) of melittin. RRMs contained pepCL9C (20-fold molar excess over rBiP) and ATP or ADP as indicated. In the final lane, KRMs were incubated with pepCL9C before fluorescence measurements (n/a, not applicable).