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Co-reconstitution of rBiP WT and sealing-incompetent rBiP. Mean KSV values (n = 3 ± SD) for NBD-pPL-sK78 RNCs bound to RRMs reconstituted with ATP and equimolar concentrations of rBiP WT and either rBiP T229G, rBiP R197H, or Kar2p measured in the absence (•) or presence (○) of melittin.
