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. 2005 Jan 31;168(3):415–427. doi: 10.1083/jcb.200407053

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Figure 3. — Some cells born in the adult cortex express CB or CR, markers of specific interneuron classes. (A) A pair of 4–5-wk-old BrdU+/CB+ neurons is shown within the box in A1 and at higher magnification color separation in A2. Pairs of BrdU+ labeled cells were frequently observed, suggesting local division from a common precursor. Note the larger BrdU−/CB+ interneuron in the lower left of A1. (B) An 11–12-wk-old BrdU+/CB+ neuron is shown within the box in the multi-channel view in B1 and in orthogonal views in B2. Note the larger BrdU−/CB+ interneuron in the upper left of B1. (C and D) 4–5-wk-old BrdU+/CR+ neurons are shown in color separations in C and (a different cell) in orthogonal views in D. In C, the BrdU+/CR+ neuron and an adjacent BrdU−/CR− cell both appear to be a satellites of a large cell on the left with a speckled nucleus visible in Hoechst staining. (E) A pair of 11–12-wk-old BrdU+/CR+ neurons are shown within the boxes in E1 and in orthogonal views in E2 and E3. Images show Z-axis projections of 6 × 4.96 μm (A1), 10 × 2.03 μm (A2), 7 × 3.17 μm (B1), 9 × 0.84 μm (B2), 14 × 1.47 μm (C), 30 × 0.64 μm (D), 6 × 2.24 μm (E1), 12 × 0.55 μm (E2), or 14 × 0.63 μm (E3). Bars: 5 μm in orthogonal views; 10 μm in all other views.