Table II. Immunostaining of BrdU-labeled cells in the adult rat neocortex and striatum.
| Age of BrdU-labeled cells
|
||||||
|---|---|---|---|---|---|---|
| Immunoreactive for | 2 h | 1–2 wk | 3–4 wk | 4–5 wk | 11–12 wk | |
| Neocortex (deep layers) | DCX | − | − | − | − | − |
| CRMP4 | na | + | + | na | na | |
| NG2 | 92.4 ± 3.8%, 140/152, n = 3 (1) | + | + | 58.5 ± 2.7%, 466/798, n = 10 (0.5) | + | |
| NeuN | − | − | na | 0.4 ± 0.13%, 33/7624, n = 10 (4) | + | |
| NeuN & NG2 | − | na | na | 0.1 ± 0.07%, 11/7624, n = 10 (4) | +/− | |
| not NeuN or NG2 | 7.6 ± 3.8%, 12/152, n = 3 (1) | na | na | 40.9 ± 2.8%, 325/798, n = 10 (0.5) | + | |
| HuC/D | na | na | na | + | na | |
| NSE | na | na | na | − | + | |
| EAAC-1 & NeuN | na | na | na | + | na | |
| GABA | na | na | na | + | + | |
| GAD-67 | na | na | na | − | + | |
| CB | na | na | na | + | + | |
| CR | na | na | na | + | + | |
| Striatum | DCX | − | + | + | + | − |
| DCX/CRMP4 | na | + | + | na | na | |
| DCX/NeuN | − | − | + | + | − | |
| NeuN | − | − | + | 5.8 ± 0.9%, 57/995, n = 8 (2) | + | |
| NSE | na | na | na | − | + | |
| GAD-67 | na | na | na | + | + | |
| CR | na | na | na | + | + | |
In cases where quantitative analysis was performed, mean % of BrdU+ cells ± SEM, d, the number of cells in category/number of BrdU+ cells analyzed, and n, the number of brains analyzed (number of sections per brain), are provided.
+, some cells of this type observed; −, not observed; na, not analyzed; +/−, very few observed.