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. 2005 Jan 31;168(3):477–488. doi: 10.1083/jcb.200407113

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — Association of SV40 with detergent-insoluble membranes. (A) Internalized SV40 but not Tf is resistant to Triton X-100 extraction at 4°C. AF488-SV40 and AF568-Tf were bound to HuH7 (left), cav-1KO (middle), and cav-1WT cells (right) on ice and allowed to internalize for the indicated times. The cells were washed, extracted for 5 min on ice with 1% Triton X-100, and fixed. Bars, 10 μm. (B) SV40 associates and internalizes with DRMs in cav-1–deficient cells. Cav-1KO cells were incubated with SV40 for 30 min at 37°C before cell lysis and extraction with 1% Triton X-100 at 4°C. Samples were floated in a linear sucrose gradient, and fractions were collected and analyzed by SDS-PAGE and immunoblotted against SV40.