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. 2005 Jan 31;168(3):477–488. doi: 10.1083/jcb.200407113

Figure 7.

Figure 7.

18 h after internalization, SV40 accumulates in the smooth ER. (A) Cav-1KO cells were incubated with AF594-SV40, fixed after 18 h, stained in indirect immunofluorescence with a Golgi marker (ManII, mannosidase II), an ER marker (CNX, Calnexin), and a marker for the smooth ER (Syn17, syntaxin 17), and analyzed by confocal microscopy. Histograms show colocalization of SV40 with the respective markers over time. Values are given as the mean ± SD. Bars, 10 μm. (B) Thin section electron micrographs of cav-1KO cells infected with SV40 for 18 h showing virus accumulations in tubulo-reticular out-growths of the smooth ER.